(+, p = 0.0015 compared to hMSC; *, p = 0.0121 compared to hMSC). BCPs constructed with cell populations labeled with DiI (red) or DiO (green), N≥3 (A), show bilaminar structure as portrayed in a schematic (B). BCPs are intended to facilitate inductive interactions (arrow) between an outer layer of male juvenile chondrocytes (XY, orange), and a core of female hMSC (XX, green). Following 21 days of culture in growth media, BCPs containing 75% hMSC and 25% JC produce as much proteoglycan as pellets containing 100% JC as determined by DMMB analysis N=10 (C). Error bars represent 95% confidence intervals.