Aoah−/− and Aoah+/+ mice were injected i.v. with 10 μg LPS per mouse as in Methods. On days 3, 7, 14 and 21, livers were perfused and harvested to isolate non-parenchymal cells (NPCs), B cells (B220+CD3−), Kupffer cells and monocyte-macrophages (F4/80+), neutrophils (CD11b+Ly-6G+), dendritic cells (CD11c+), T cells (CD3+), NK cells (CD3-NK1.1+). Cell densities were calculated as follows: (Percentage of cell type X total NPCs number)/liver weight (g)). Open squares = Aoah−/−, closed circles = Aoah+/+. n = 3 mice/time point. Significance of differences between Aoah+/+ and Aoah−/− mice: * = p < 0.05, ** = p <0.01, *** = p < 0.001.