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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Invest Dermatol. 2011 Jun 30;131(11):2306–2315. doi: 10.1038/jid.2011.188

Figure 1. Loss of E-cadherin function is associated with up-regulation of FAK and Src expression and/or phosphorylation.

Figure 1

WB analysis of lysates of H-2Kd-Ecad-II-4 cells revealed an increase in the expression and specific tyrosine phosphorylation of FAK (a). qRT-PCR analysis revealed a significant increase in FAK mRNA expression in H-2Kd-Ecad-II-4, relative to its level in II-4 cells. The mean ± SD of FAK or Src mRNA expression levels in H-2Kd-Ecad-II-4 relative to II-4 cells are shown (b). WB analysis showed similar expression level of Src protein in both cell lines (c). WB and ratio analysis of cell fractions of the two cell lines revealed increased tyrosine phosphorylation of Src at Tyr416 in the membrane fraction of H-2Kd-Ecad-II-4 cells (d). NS, non-significant.