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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Invest Dermatol. 2011 Jun 30;131(11):2306–2315. doi: 10.1038/jid.2011.188

Figure 5. Decreased FAK or Src in H-2Kd-Ecad-II-4 cells is associated with up-regulation and stabilization of E-cadherin protein leading to normalization of tissue phenotype.

Figure 5

Immunofluorescence staining of sh-Scrambled-H-2Kd-Ecad-II-4 tissue sections showed inconsistent E-cadherin staining and cell invasion (a, arrow, inset), and while K1 was not detected (d), Ki-67-positive cells appeared throughout the epidermis (g, arrowheads). sh-FAK-H-2Kd-Ecad-II-4 tissues showed intense E-cadherin staining (b), K1 expression (e) and Ki67-positive cells at the basal and immediate suprabasal layers (h). sh-Src-H-2Kd-Ecad-II-4 tissues revealed intense E-cadherin staining (c), elevated K1 (f), and Ki67-positive cells at the basal layer (i). Bar, 100µm. While an increase in E-cadherin protein was detected in sh-FAK- or sh-Src-H-2Kd-Ecad-II-4 cells (j), E-cadherin mRNA level was not significantly changed (k). Representative WB of E-cadherin in Cycloheximide-treated cultures (l), and the mean ± SD of the remaining E-cadherin in these cultures in 5 independent experiments (m).