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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Pediatr Gastroenterol Nutr. 2011 Oct;53(4):371–379. doi: 10.1097/MPG.0b013e318219c397

7. Bacterial overgrowth of the CF mouse small intestine and effects of exogenous IAP and L-Phe.

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The small intestine was resected and flushed with PBS containing a mucolytic (10 mM dithiothreitol). The flushed fluid was centrifuged and the pellets processed to extract bacterial DNA for realtime PCR of the bacterial 16S gene. The copy numbers of the bacterial 16S rRNA gene were used to estimate bacterial load per intestine. Con: Control, mice were fed the liquid diet; IAP: Mice were fed the liquid diet supplemented with 13.3 U/ml purified calf IAP; L-Phe: Mice were fed the liquid diet supplemented with 10 mM L-Phe. (*) p<0.0001 CF vs corresponding WT; (+) p=0.00027 CF L-Phe vs CF Con (n=5 WT Con, 6 WT IAP, 10 WT L-Phe, 6 CF Con, 6 CF IAP, 4 CF L-Phe)