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. Author manuscript; available in PMC: 2012 Mar 22.
Published in final edited form as: Nature. 2011 Sep 21;477(7365):482–485. doi: 10.1038/nature10296

Figure 2. Drosophila: Absence of effects of dSir2 on lifespan.

Figure 2

All lines were outcrossed into wDah (+/+) a, Lifespan in flies over-expressing dSir2EP2300 driven via tubulin-GAL4 (tub-GAL4) is longer than wild type, but not than the tubulin-GAL4 /+ genetic control. Median lifespans: +/+ = 39 days, dSir2EP2300/tubulin-GAL4 = 59 days, dSir2EP2300/+ = 53 days, tubulin-GAL4 /+ = 60 days. dSir2EP2300/tubulin-GAL4 vs. dSir2EP2300/+, P = 0.0006; dSir2EP2300/tubulin-GAL4 vs. tubulin-GAL4 /+, P = 0.9295; dSir2EP2300/tubulin-GAL4 vs. +/+, P <0.0001 . b, Lifespan in flies over-expressing dSir2-Myc9 is longer than in wild type, but not than in the tubulin-GAL4 control. Median lifespans: +/+ = 39 days, dSir2-Myc9/tubulin-GAL4 = 67, dSir2-Myc9/+ = 41 days, tubulin-GAL4/+ = 60 days. dSir2-Myc9/tubulin-GAL4 vs. dSir2-Myc9/+, P = 0.0001; dSir2-Myc9/tubulin-GAL4 vs. tubulin-GAL4/+, P = 0.1354; dSir2-Myc9/tubulin-GAL4 vs. +/+, P <0.0001. Compared using log rank test, n=200. c, Effect of dietary restriction on Drosophila lifespan is not dSir2 dependent. Flies were assayed over five concentrations of SYA media and data are presented as the median lifespan on each food concentration. All lines were outcrossed into Canton S (+/+). P values confirm that all flies respond normally to DR when median lifespans are compared for DR vs. fully-fed (FF) conditions30.