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. Author manuscript; available in PMC: 2011 Oct 6.
Published in final edited form as: Crit Care Med. 2010 Feb;38(2):562–571. doi: 10.1097/CCM.0b013e3181cb10b3

Fig. 9. O-GlcNAcylation and phosphorylation in the Liver tissue extracts 24 hours after hypovolemic shock and resuscitation.

Fig. 9

Fig. 9

Tissue samples were separated on 10% gels. Membranes were incubated with A) O-GlcNAc (CTD 110.6) antibody, B) O-GlcNAc antibody + 10 mM GlcNAc, C) Phosphoserine or D) Phosphothreonine antibodies. The representative blots show two-two selected samples of each group. Bar charts represent the mean area densities of E) O-GlcNAc, F) Phosphoserine and G) Phosphothreonine immunoblots, related to Sham animals. Mean densities were normalized to β-actin staining. Data are shown on bar charts as mean ± SEM, n=7 in each group. ANOVA, * p<0.05 Vs Sham,# p<0.05 Vs Control and† p<0.05 Vs GlcN group.