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. Author manuscript; available in PMC: 2011 Oct 6.
Published in final edited form as: Cell Cycle. 2009 Dec 14;8(23):3904–3913. doi: 10.4161/cc.8.23.10074

Figure 5.

Figure 5

embryos overexpressing CDC14B that are arrested at the 1-cell stage have low CDC2A activity. (A) one-cell embryos were collected at the indicated cell cycle stage and CDC2A and MApK activities for histone H1 or MBp, respectively, were measured by in vitro kinase assays. The graph represents quantification of the band intensity as measured in Image J for CDC2A of three independent experiments. (B) Immunoblotting analysis of levels of CCNB1 protein between control embryos in G2 phase to 1-cell arrested embryos that were overexpressing CDC14B. this experiment was conducted two times. (C) embryos overexpressing CDC14B that did not contain visible pronuclear membranes were fixed in 3.7% paraformaldehyde and stained with an antibody against α-tubulin to visualize the spindles and mounted in propidium iodide to visualize the DNA. the images were obtained on a laser-scanning confocal microscope.