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. Author manuscript; available in PMC: 2012 Oct 4.

Published in final edited form as: Biochemistry. 2011 Sep 8;50(39):8463–8477. doi: 10.1021/bi201007t

These spectra show through-space coupling between guanine N1 and thymine N3 imino protons in neighboring base pairs for the (A) parent duplex 1–2 and (B) 8oxoG lesion duplex 1oxo-2. Crosspeaks in this region were assigned by tracing them consecutively from one end of the helix (G₂₉) to the other (G₁₄), as shown with solid lines. Thymine imino proton resonances are found between 13.5–14 ppm, and guanine imino proton resonances between 12.4–12.9 ppm, so the peaks furthest from the diagonal correspond to G-T and T-G steps; peaks close to the diagonal correspond to G-G or T-T steps. Note that the terminal guanine imino protons exchange too quickly to be seen on the timescale of this NOESY experiment.

These spectra show through-space coupling between guanine N1 and thymine N3 imino protons in neighboring base pairs for the (A) parent duplex 1–2 and (B) 8oxoG lesion duplex 1oxo-2. Crosspeaks in this region were assigned by tracing them consecutively from one end of the helix (G₂₉) to the other (G₁₄), as shown with solid lines. Thymine imino proton resonances are found between 13.5–14 ppm, and guanine imino proton resonances between 12.4–12.9 ppm, so the peaks furthest from the diagonal correspond to G-T and T-G steps; peaks close to the diagonal correspond to G-G or T-T steps. Note that the terminal guanine imino protons exchange too quickly to be seen on the timescale of this NOESY experiment.