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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Bioorg Med Chem. 2011 Aug 16;19(19):5886–5895. doi: 10.1016/j.bmc.2011.08.012

Fig. 2.

Fig. 2

Purification of E. coli CDP-ME kinase. Over-expression of E. coli CDP-ME kinase was induced in E. coli HMS174 cells harboring the plasmid expressing the E. coli IspE gene. The overproduced CDP-ME kinase seen in the lysate of the bacteria (marked by ⊕ in lane 2) was purified by Nickel affinity chromatography and collected in the eluate (also marked by ⊕). FT: Flow-through.