. Author manuscript; available in PMC: 2011 Oct 6.

Published in final edited form as: J Biol Chem. 2005 Mar 21;280(22):20987–20994. doi: 10.1074/jbc.M409639200

Table I. Purification of the acetylcholine receptor protein.

This semi-preparative purification is based on a starting amount of 28g of heads (300 g of flies).

Fraction	¹²⁵I-α-Bungarotoxin binding	Recovery	Protein	Specific activity	Purification fold

	pmol	%	mg	μmol/g protein
Homogenate	2281	100	3326	0.00067
Triton X-100 extract	453	19	273	0.00166	2.4
α-Cobratoxin column eluate	210	9.2	1.3	0.156	232
Lectin column eluate	106	4.6	0.056	1.9	2830
YM100 concentrate	45	2.0	0.0113	3.9	5820