. 2011 Oct 6;7(10):e1002171. doi: 10.1371/journal.pcbi.1002171

Table 1. The affinities of the stem-loop constructs employed here.

	K_d^probe (nM)^a	K_d^dep (nM)^b
0GC	13 (±2)	5.2
1GC	42 (±4)	6.6
2GC	310 (±20)	11.3
3GC	2,600 (±40)	36.9
4GC	not used	225.8
5GC	not used	3,647

^aFAM/BHQ-1 modified molecular beacons are ∼8 kJ more stable than their unlabeled stem-loop counterparts due to interactions between the dye and the quencher [47]; their affinities for a 13-base sequence targeting the loop have been determined as reported elsewhere [33]. ^b K_d of the unlabeled depletant constructs were calculated using their simulated switching equilibrium and the expected K_d of the open form of the molecular beacon [33].