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. 2011 Oct 6;7(10):e1002280. doi: 10.1371/journal.ppat.1002280

Figure 8. Expression of degradation domain (DD)-tagged TgACTI alleles in Toxoplasma.

Figure 8

(A) Expression of DD-TgACTI fusion proteins following treatment ± Shield-1 for 40 hr and detected by Western blot with anti-TgACTI antibody. All strains express the endogenous TgACTI while the fusion proteins (DD-TgACTI) were only expressed by the transfected strains in the presence of Shield-1. (B) Expression of DD-tagged TgACTI alleles following treatment ± Shield-1 for 24 hr and stained for immunofluorescence with anti-SAG1 (surface antigen 1, red) and anti-c-myc (green) to detect the DD-TgACTI fusion protein. (C) Effects of DD-TgACTI allele expression on plaque formation. HFF monolayers were infected with untransfected parasites or those expressing DD-TgACTI alleles ± Shield-1 for 7 days and visualized by crystal violet staining. Representative of three or more similar experiments.