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. 2011 Oct 6;7(10):e1002280. doi: 10.1371/journal.ppat.1002280

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Skillman et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative composite videos of normal gliding motility by parasites expressing DD-wild type TgACTI. (B,C) Representative composite videos of gliding by parasites expressing stabilized actin mutants revealed examples of stalled, incomplete or off-track circular patterns and disrupted helical patterns that were classified as aberrant. For helical gliding, the number of complete turns made during the time-lapse sequence are numbered. Images are composite frames from 60 sec of video recording. Scale bars, 5 µm. (D) Quantification of number of parasites undergoing each category of gliding motility following treatment ± Shield-1 for 40 hr. The percentage of parasites undergoing aberrant gliding (as defined in B,C) increased in the presence of Shield-1 in the mutant actins as compared to wild type, * P<0.05 ** P<0.01, (Student's t-test). Mean ± S.D. (E) Comparison of radii of circular tracks formed during normal gliding by DD- wild type expressing parasites vs. parasites expressing mutant actins that formed aberrant circular tracks (i.e. stalled or off-track). * P<0.001 ** P<0.0001 (Mann-Whitney test). Mean shown by horizontal line. (F) Comparison of parasite curvature during normal gliding by DD-wild type expressing parasites vs. parasites expressing mutant actins that formed aberrant circular tracks (i.e. stalled or off-track). Data shown are the average curvature radii of individual parasites during gliding motility. (G) Quantification of number of parasites undergoing each category of gliding motility following treatment ± Shield-1 for 6 hr. The percentage of parasites undergoing aberrant gliding (as defined in B, C) increased in the presence of Shield-1 in the mutant actins as compared to wild type, * P<0.001, (Student's t-test). Mean ± S.D.