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. 2011 Oct 6;7(10):e1002291. doi: 10.1371/journal.ppat.1002291

Figure 6. Characterization of SCORD5 (At1g64550).

Figure 6

(A) The intron/exon structure of At1g64550 and T-DNA insertion sites in scord5 (scord5-1) and 188G03 (scord5-2) mutants. Locations of primers used in this study are indicated. (B) and (D) PCR reactions using genomic DNA from Col-7 and scord5 (scord5-1) (B) or 188G03 (scord5-2) (D) as template. (C) and (E) RT-PCR reactions using total RNA from Col-7 and scord5 (scord5-1) (C) or 188G03 (scord5-2) (E) as template. The RT-PCR product of ACT1 (Arabidopsis actin 1; At2g37620) was used as a loading control. (F) Stomata apertures of Col-0, scord5-1, and scord5-2 leaf peels incubated with H2O or Pst DC3118 (1×108 CFU/ml) for 1 h. (G) Stomata apertures of Col-7, scord5-1 and scord5 mutant plants complemented with a 5.4-kb-genomic region of At1g64550, in response to Pst DC3118 (1×108 CFU/ml) for 1 h.