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. 2011 Oct 6;6(10):e24832. doi: 10.1371/journal.pone.0024832

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IHC stains were subjectively scored on a 0–4 scale (0 = none; 1 = minimal; 2 = mild; 3 = moderate; 4 = marked). For each tissue type, IHC scores for all 3 animals from the same dose and necropsy day group were summed. If, for an individual animal, more than one slide of the same tissue type was examined and scored, the average score for that tissue type was used in the summation.