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. 2011 Oct 6;6(10):e25714. doi: 10.1371/journal.pone.0025714

Figure 4. Rate of vesicle replenishment becomes highly sensitive to intracellular EGTA with maturation.

Figure 4

A) Two paired-pulse recordings of ICa and ΔCm in response to two consecutive 100 ms depolarizing steps (as in Fig 3) separated by either 200 ms or 1 s in an E12 apical tall HC using 2 mM intracellular EGTA. (B) Two paired-pulse recordings of ICa and ΔCm as in A but in mature P2 apical HCs. Note the strong depression in ΔCm after the 2nd pulse. (C) Comparative RRP recovery after 200 ms inter-pulse using 0.5 or 2 mM intracellular EGTA in E12 (n = 5) and P2 (n = 5) apical HCs. Asterisk indicates statistical significance with p<0.01. (D) Comparative kinetics of RRP recovery using 0.5 or 2 mM intracellular EGTA in mature P2 (n = 5) or immature E12 (n = 5) apical HCs.