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. 2011 Oct 6;6(10):e26032. doi: 10.1371/journal.pone.0026032

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Antonelou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative immunoblot analysis of purified membrane and cytosol fractions of untreated RBCs (-); RBCs treated with NP-40 (NP-40) or RBCs digested with proteinase K in the absence (PrK) or presence of NP-40 (PrK/NP-40). Samples (N = 3) were probed with specific anti-sCLU and anti-Band 3 antibodies. (B) Immunobloting of streptavidin, sCLU and Band 3 in purified membrane and cytosolic fractions of control (Co) or biotinylated (BIO) RBCs (N = 3). Molecular weight markers are shown to the right of the blots.