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. 2011 Oct 6;6(10):e25984. doi: 10.1371/journal.pone.0025984

Figure 6. Effects of soluble recombinant Steel factor on PGC motility in wild type embryos at E11.0.

Figure 6

(Column A-C) Frames at t = 0, t = 6 and t = 12 hours respectively from movies of E11.0 wild type embryo slices with or without addition of 200 ng/ml soluble recombinant Steel factor. (D) The relative expression level of membrane-bound and soluble Steel factor mRNA in E10.5 genital ridges, E11.5 genital ridges and E11.5 midline mysenchyme as determined by real-time RT-PCR. *p<0.05, **p<0.01. (E and F) E-cadherin expression in PGCs without (E) or with (F) addition of Steel factor. Upper panels show E-cadherin (red) staining. The lower panels show the merged images with PGC marker Stella-GFP (green). No difference in expression of E-cadherin was observed between groups.