Fig. 3.

I56i enhancer activity is impaired by the 182-SNP in migrating neuronal precursors at E13.5. (A-H) Immunolocalization of β-galactosidase (red) in I56i-lacZ (A-D) and vI56i-lacZ (E-H) transgenic mouse brains at the level of the LGE/MGE (A,B,E,F) and CGE (C,D,G,H). The boxed regions in A,C,E,G are shown at higher magnification in B,D,F,H. Numerous lacZ-expressing neurons were observed migrating from the MGE and CGE towards the developing cortex in I56i-lacZ brains (A-D), whereas only a few scattered lacZ-expressing cells were detected in the migrating streams leaving both eminences from the vI56i-lacZ brains (E-H). Scale bars: 500 μm in A,C,E,G; 250 μm in B,D,F,H.