Fig. 2.
Mesothelial and epithelial FGF9 differentially affect mesenchymal and epithelial proliferation. (A-F) BrdU labeling of control (A,D), Fgf9Dermo1 (B,E) and Fgf9Shh (C,F) mouse lung showing decreased proliferation in the mesenchyme of the Fgf9Dermo1 lung and the epithelium of the Fgf9Shh lung at E12.5 and E14.5. Note that at E14.5 proliferation was decreased in both the epithelium and mesenchyme in Fgf9Dermo1 and Fgf9Shh lung. (G,H) Quantitation of BrdU labeling at E12.5 (G) and E14.5 (H). *P<0.01 (Student's t-test). Error bars represent s.d. (I-K) TUNEL labeling of control (I), Fgf9Dermo1 (J) and Fgf9Shh (K) lung at E12.5 showing no difference in rates of cell death. Scale bars: 50 μm.