Fig. 5.

Regulation of BMP pathways by mesothelial and epithelial FGF9. (A-C) Expression of Bmp4 in control (A), Fgf9Dermo1 (B) and Fgf9Shh (C) mouse lung mesenchyme at E12.5. (D-F) Expression of Nog in control (D), Fgf9Dermo1 (E) and Fgf9Shh (F) lung epithelium at E12.5. Bmp4 expression was similar but Nog expression was significantly increased in both Fgf9Dermo1 and Fgf9Shh lungs. (G-I) Frozen sections of the whole mounts shown in D-F. (J) Quantitative RT-PCR detection of Nog expression showing increased expression in Fgf9Dermo1 and Fgf9Shh E12.5 lung compared with control. Error bars represent s.d. *P<0.05, **P<0.01, Student's t-test. Scale bar: 200 μm for A-F; 25 μm for G-I.