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Comparing different steps followed in manual and reciprocating methods for developing IgG microarrays.
| Manual | Reciprocating | |
|---|---|---|
| Blocking buffer | 30 min on a rocker | 1 min |
| Goat anti-human IgG conjugated with AP | 60 min on a rocker | Varying (2.5, 5, 7, or 20 min) |
| TTBS | Three quick washes and 3 washes of 5 min | 2.5 min |
| TBS | Three quick washes and 3 washes of 5 min | 1.5 min |
| NBT∕BCIP substrate | 4 min | 3.5 min |
| DI water | 5 min | 1.5 min |
