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. Author manuscript; available in PMC: 2012 Oct 13.
Published in final edited form as: J Med Chem. 2011 Sep 9;54(19):6647–6656. doi: 10.1021/jm200521a

Figure 4.

Figure 4

Prodrug 14 is cell permeable (A) and undergoes intracellular activation in A549 cells to release bisphosphonate 1 (B). Figure 4A: ion chromatograms showing absence of prodrug 14 in untreated cells (a), and detection of 14 (68.6 ng/104 cells) in cells treated with 10 µM 14 (b) and cells treated with 30 µM 14 (169.3 ng/104 cells) (c). Figure 4B: ion chromatograms showing absence of tetra-silyl bisphosphonate 18 following MTBSTFA reaction of lysate from untreated cells (d), and detection of 18 in cells treated with 30 µM bisphosphonate 1 (e), cells treated with 10 µM prodrug 14 (f) and cells treated with 30 µM prodrug 14. Ion chromatograms are offset in the y-dimension for clarity.