Fig. 3.

GLP-1 modulates iNKT cell cytokines. IFN-γ (a) and IL-4 (b) production by iNKT cells (1 × 10⁵) after 24 h co-culture at a 1:1 ratio with CD1d-transfected C1R cells unloaded in the absence or presence of GLP-1 (150 μg/ml) (n = 5). IFN-γ (c) and IL-4 (d) production by iNKT cells after 24 h co-culture with CD1d-transfected C1R cells loaded or unloaded with 100 ng/ml of αGalCer in presence of increasing concentrations of GLP-1 (0–150 μg/ml, n = 3). Cytokine concentrations were measured by ELISA and analysed using GraphPad Prism (n = 3). *p < 0.05, ^† p < 0.005, ^‡ p < 0.0001