Figure 5.
The adenovirus-resistant phenotype is sustained following transplantation into new mice. (a) Severe combined immunodeficiency (SCID) mice (n = 10) were xenografted intraperitoneally (i.p.) with 5 × 106 luciferase expressing SKOV3-Luc cells and were treated i.p. with total of 3 × 109 viral particle (VP) of Ad5/3-d24 or PBS (black arrows). Tumor growth was monitored by in vivo bioluminescence imaging. (a) On day 27 relapsed and untreated i.p. tumors were surgically removed and (b) reimplanted, with tumor stroma, into a second round of mice (n = 5–7). Following identical treatment (total 3 × 109 VP of Ad5/3-d24 or PBS; black arrows), virus-resistant tumors showed no inhibition of tumor growth, while susceptible tumors responded to treatment (P ≤ 0.001) (b). Each data point represents the mean ± SEM.