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. 2011 Aug 2;19(10):1826–1832. doi: 10.1038/mt.2011.154

Figure 1.

Figure 1

Adeno-associated virus serotype-9 (AAV-9) microdystrophin vector efficiently transduced the myocardium of aged mdx mice. (a) Representative Dys-3 immunofluorescence staining of whole mdx heart infected with AAV-9 microdystrophin vector. (b) Representative high-power images of immunofluorescence staining from untreated mdx and AAV-9 microdystrophin vector-treated mdx. Top panel: Dys-3 antibody immunostaining. Dys-3 is an antibody that specifically recognizes human dystrophin (Hum Dys). Dys-3 immunostaining reveals the expression of human-derived microdystrophin. Middle panel: Mandys-8 antibody (R11) immunostaining. Mandys-8 antibody specifically reacts with spectrin-like repeat 11, a repeat deleted in microdystrophin. Bottom panel: Utrophin immunostaining. (c) Representative western blot analysis of dystrophin expression in BL10, untreated mdx and AAV-infected mdx mice. Top panel: western blot with the DysB antibody; bottom panel: Rapid blue staining of a duplicated gel. µ-Dys, microdystrophin. Arrowhead, full-length mouse dystrophin; *Human ΔR4-23/ΔC microdystrophin.