Figure 1.

Intranasal administration of exosomes results in localization to the brain. A total of 10 µg of IRDye 800-labeled exosomes (green) released from different types of cells as listed or DIR dye-labeled EL-4 exosomes (b,c) were administered intranasally into C57BL/6j mice. (a) 30 minutes or at different time points (d) postintranasal administration, the brain was cut sagittally, and the ventral sides of cut brain were placed against the scanner for imaging using the (a,d) Odyssey laser-scanning imager or (b) whole mice or (c) each organ were imaged using the Carestream Molecular Imaging System. Representative sagittal images from the center of the brain (n = 5; a,d), images of whole body (b), or images of each organ (n = 5; c) are shown. Results were obtained from three independent experiments with five mice in each group of mice.