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Immunoblot Analysis of the Oxoacid Dehydrogenases and Respiratory Chain Subunits in NFU1, BOLA3, and ISCU Mutant Fibroblasts
Immunoblots of 25 μg fibroblast mitochondria were immunoblotted with antibodies against NFU1, lipoate, complex I subunits (NDUFS1, NDUFV1, NDUFS2, NDUFA9, and NDUFA8), complex II subunit SDHA, complex III subunits (UQCRFS1, UQCRC1, and CYTB), and citrate synthase (CS).
