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. 2011 Jun 1;17(9-10):1022–1030. doi: 10.2119/molmed.2011.00163

Figure 4.

Figure 4

Effect of RSVA314 and RSVA405 treatment on AMPK activation at the different phases of adipocyte differentiation. (A–C) Differentiating 3T3-L1 cells were treated with the indicated concentrations of RSVA314 and RSVA405 or vehicle (DMSO; CTRL). Phospho-AMPK, total AMPK, phospho-ACC, total ACC and actin protein levels were examined on day 0, 2, 4 and 8 of adipocyte differentiation (Diff. days; A). Densitometric analyses and quantification of the levels of phospho-AMPK and phospho-ACC at the different phases of adipocyte differentiation in control cells (B) and on day 2 of differentiation in cells treated as in (A) were performed (C). a.u., arbitrary units.