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AAT inhibits neutrophil calpain I activity. Dose-dependent inhibitory effects of AAT on the enzymatic activity (expressed as relative fluorescence units) of calpain I on the specific substrate Ac-LLY-AFC in human neutrophils are shown. The fluorescence value of all samples is corrected by subtracting the value of the blank and then calculating the mean fluorescence value (mean ± SEM) for each sample from duplicate readings from six independent experiments.
