Figure 6.

AAT increases intracellular cholesterol levels. Cells were incubated with AAT for the indicated time periods. The intra-cellular cholesterol was stained with filipin, as described in the Materials and Methods. Filipin fluorescence appears shortly after stimulation with AAT and enhances in a time-dependent manner. Images were acquired with constant exposure time and under the same magnification for the whole series. Representative images of three independent experiments are shown. Scale bar = 20 μm.