Figure 1.

The protein kinase C inhibitor Gö6976 (Gö), but not GF-109203X (GFX), inhibits C-type natriuretic peptide (CNP)-dependent activation of GC-B in whole cells. A. The structures of GF-109203X and Gö6976 are shown. B. 293T-GC-B cells were incubated with or without the indicated compounds for 30 min at 37°C and then cells were stimulated with 1 µM CNP for 1 min and intracellular cGMP concentrations were determined. **P < 0.02; ***P < 0.001, compared with cGMP concentrations measured in cells treated with CNP alone; n = 6 from two experiments. PMA, phorbol 12-myristate 13-acetate.