Figure 3.

Gö6976 is a potent and rapid inhibitor of GC-B. A. 293T-GC-B cells were incubated with increasing concentrations of Gö6976 for 1 h at 37°C and then the cells were stimulated with 1 µM C-type natriuretic peptide (CNP) for 1 min and cGMP accumulation was determined and plotted as a function of log (Gö6976 concentration). IC₅₀ = 380 nM, r² = 0.81, n = 6 from two experiments. B. 293T-GCB cells were treated with 10 µM Gö6976 for the indicated periods of time and then stimulated with 1 µM CNP for 1 min. cGMP accumulation was measured and plotted as a function of time in the presence of Gö6976. t_½ = 7 s, r² = 0.82, n≥ 3 from two experiments. ^ indicates an n of 3, for all other points n = 6.