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. 2011 Sep;164(2b):406–418. doi: 10.1111/j.1476-5381.2010.01120.x

Figure 1.

Figure 1

Iron loading and erythrophagocytosis by RAW 264.7 macrophages: effects on wild-type (WT) and iron-sensitive (IS) cells. (A) Cells were incubated with ferric ammonium citrate (FAC) at the indicated concentrations in the absence or presence of 1 µM hepcidin and cell viability was determined after 24 h with the fluorescent redox probe Alamar Blue. Results are shown as means ± standard deviation (SD) (n = 5) of Alamar Blue's fluorescence for the indicated treatment relative to the respective untreated controls. (B) Cells were incubated for 6–8 h with Venofer (V; 500 µM Fe) in the absence or presence of 1 µM hepcidin (+Hep), and subsequently washed and cultured for 18 additional h and analysed as described in (A). Results shown are relative to the respective untreated controls. (C) Cells were incubated for 1 h with or without opsonized erythrocytes (+RBC) as described in the Methods section, and then cultured overnight with or without 1 µM hepcidin (+Hep). Cell viability was measured with Alamar Blue and is shown relative to respective untreated controls ± SD (*P < 0.05).