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. 2011 Sep;164(2b):406–418. doi: 10.1111/j.1476-5381.2010.01120.x

Figure 6.

Figure 6

Effect of chelation on ferritin levels in iron-loaded RAW 264.7 (IS) macrophages. Cells were (A) incubated overnight with 100 µM FAC, or (B) for 1 h with opsonized erythrocytes [red blood cells (RBCs)], then washed and grown for 24 h in the presence of deferiprone (DFP), deferasirox (DFR) or deferrioxamine (DFO), given in micromolar. Ferritin levels in whole cell lysates were determined by Western blotting with antibodies against H-ferritin and blots were re-probed for actin. Relative values of ferritin levels (given as % of control) determined by densitometry are shown. Data are representative of one out of four independent experiments.