Table 1.

Non-haeme iron content in RAW 264.7 WT and IS cells following iron loading

Treatment	Haeme content
Treatment	WT	IS
Basal (-FAC)	0.49 ± 0.09	0.67 ± 0.05
FAC	1.61 ± 0.09*	3.24 ± 0.11*,**
FAC + Hep	1.84 ± 0.05	4.27 ± 0.7
Basal (-V)	0.43 ± 0.06	0.43 ± 0.04
V	3.82 ± 0.20*	2.91 ± 0.49**
V + hep	4.87 ± 0.10	3.66 ± 0.27
Basal (-RBC)	0.55 ± 0.04*	0.66 ± 0.05
RBC	0.83 ± 0.02*	1.22 ± 0.34*,**
RBC + hep	0.96 ± 0.12	1.20 ± 0.23

WT and IS cells were incubated: (a) for 1 h with opsonized erythrocytes [red blood cells (RBCs)] and then cultured overnight without or with 1 µM hepcidin (+Hep) or (b) for overnight in regular culture medium, or in medium supplemented with either 100 µM ferric ammonium citrate (FAC) ± 1 µM hepcidin (FAC + Hep) or with Venofer (V; 500 µM Fe) ± 1 µM hepcidin (hep).

The total non-haeme iron content (nmol·µg⁻¹ protein) in untreated cells (basal levels) and iron loaded cells was determined after extensive washing of the cells as described in the Methods section and is given as mean values ± standard deviation with ^* denoting significant difference at P < 0.05 from the respective control (indicated as basal) ^** significant difference between iron sensitive (IS) and wild type (WT) for the equivalent treatment.