Table 2.

Protein carbonyl (CO) groups in RAW 264.7 wild type (WT) and iron sensitive (IS) cells following iron loading

	Cell CO groups (nmol·mg−1 protein ± SD)
RAW264.7 cell type	Basal	FAC	FAC + Hep	V	V + Hep
WT	0.57 ± 0.09	0.68 ± 0.2	0.71 ± 0.3	0.75 ± 0.11	0.84 ± 0.13**
IS	0.62 ± 0.21	1.97 ± 0.31*,**	2.57 ± 0.30*,**	1.97 ± 0.33*,**	2.86 ± 0.47*,**

The CO group levels in basal growth conditions and following 48 exposure to 100 µM FAC or Venofer (V) (500 µM Fe) ± hepcidin (Hep) (1 µg·mL⁻¹) were determined with the 2,4-dinitrophenyl-hydrazine assay as described in the Methods section.

The indicated values represent means of three equivalent experiments ± SD and significant differences (at P < 0.05) between IS and WT cells for a given treatment are indicated as ^* and between treatment and basal conditions for a given cell type as ^**.