Table 1.
Basal resistance and changes in baseline Isc levels following addition of TTX or DPP IV inhibitor (Compound 3) to basolateral surface of colon mucosa from human and mouse tissue
| Resistance (Ω·cm2) | Basal Isc (µA·cm−2) | 100 nM TTX (µA·cm−2) | 1 µM Compound 3 (µA·cm−2) | |
|---|---|---|---|---|
| Human colon | 82.2 ± 4.0 (37) | 60.2 ± 5.6 (37) | −17.9 ± 3.4 (7) | 2.8 ± 1.0 (8) |
| Mouse jejunum | ||||
| WT | 24.4 ± 1.1 (6) | 82.4 ± 13.0 (6) | ND | ND |
| Mouse colon | ||||
| WT | 34.2 ± 1.8 (23) | 55.7 ± 5.7 (70) | −3.9 ± 1.3 (17) | −1.3 ± 0.3 (6) |
| NPY−/− | 30.1 ± 3.1 (18) | 80.8 ± 13.1 (18) | −9.4 ± 1.8 (7) | 0.4 ± 0.2 (8)* |
| PYY−/− | 40.0 ± 2.9 (20) | 66.5 ± 9.8 (20) | −8.6 ± 2.8 (6) | −1.3 ± 0.5 (8) |
| NPYPYY−/− | 24.4 ± 3.3 (8) | 49.6 ± 15.6 (8) | −13.3 ± 3.5 (7)** | ND |
Each value is the mean ± SEM from the number of observations shown in parenthesis.
*
P≤ 0.05
**
P≤ 0.01 compared with mouse wild-type (WT) controls. ND, not determined.