SDS-PAGE of antigen from formulations. (Lane 1) Mol. wt. markers: (A) phosphorylase b, 97.4 kDa; (B) BSA, 66.2 kDa; (C) ovalbumin, 45.0 kDa; (D) bovine carbonic anhydrase, 31.0 kDa; (E) trypsin inhibitor, 21.5 kDa; (F) lysozyme, 14.2 kDa. (Lane 2) native DTx solution. (Lane3) Alum-adsorbed DTx. (Lane 4) DTx nano-bilosomal formulation.