Table 2.

Effect of mGlu₂ NAMs on [³H]-LY354740 saturation binding at mGlu₂

	Kd (nM)	Bmax (fmol·mg−1)
LY354740	9.1 ± 0.9	14632 ± 151
+10 nM RO4988546	12.3 ± 2.0	13540 ± 420
+30 nM RO4988546	26.0 ± 1.5	13870 ± 170
+100 nM RO4988546	39.7 ± 3.2	13175 ± 515
+2 nM RO5488608	14.6 ± 1.1	14830 ± 410
+3.16 nM RO5488608	11.1 ± 0.3	11515 ± 185
+5 nM RO5488608	21.5 ± 1.0	9034 ± 150
+10 nM RO5488608	24.1 ± 1.4	7465 ± 171

Calculated K_d and B_max values for the saturation isotherms of [³H]-LY354740 in the absence and presence of mGlu₂ NAMs shown in Figure 3. Values are mean ± SEM from individual experiments performed in duplicate (LY354740 n = 8, RO4988546 n = 2, RO5488608 3.16 nM and 10 nM n = 2, RO5488608 2 nM and 5 nM n = 3).