Table 1.
Radioligand binding data for expressed receptors
| Receptor | 3H-ligand Kd (pM) | Bmax (fmol mg-1 protein) |
|---|---|---|
| Adrenoceptor | ||
| [3H]-prazosin | ||
| α1A | 234 ± 31 | 2191 ± 53 |
| α1B | 181 ± 28 | 1946 ± 59 |
| α1D | 148 ± 31 | 438 ± 18 |
| [3H]-MK-912 | ||
| α2A | 876 ± 128 | 5190 ± 141 |
| α2B | 599 ± 134 | 10343 ± 409 |
| α2C | 86 ± 32 | 1101 ± 186 |
| [3H]-CGP-12177 | ||
| β1 | 664 ± 189 | 2910 ± 249 |
| β2 | 738 ± 204 | 2385 ± 204 |
| mAChR | [3H]-NMS | |
| M1 | 460 ± 78 | 3162 ± 115 |
| M4 | 190 ± 34 | 888 ± 35 |
Saturation binding experiments were performed using crude membrane preparations of infected Sf9 cells. Data are mean values ± SD from one batch of membranes analysed in two separate experiments.