Figure 6.

Effect of SPA0355 on RANKL-induced osteoclast differentiation. (A) BMMs were cultured for 4 days with M-CSF (30 ng·mL⁻¹) and RANKL (50 ng·mL⁻¹) in the presence of the indicated concentrations of SPA0355. Cells were fixed with 3.7% formalin, permeabilized with 0.1% Triton X-100 and stained with TRAP solution. (B) TRAP-positive cells were counted as osteoclasts. Each value represents the mean ± SEM of the three independent experiments. **P < 0.01, significantly different from untreated control.