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. 2011 Aug 9;105(5):687–693. doi: 10.1038/bjc.2011.306

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Copyright © 2011 Cancer Research UK

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Effect of BCG on the active status of immune cells and cytokine production. Peripheral-blood mononuclear cells harvested from pathologically normal subjects were exposed to BCG for up to 72 h before assessing the percentage of CD69-expressing cells by nested flow cytometric analyses. The gating profile (A) allowed for identification of five immune subsets, and the percentage of CD69 positive cells assessed (B). The amounts of six cytokines produced by PBMCs in response to BCG were also assessed (C). Each data column represents the mean and s.d. of five separate individuals at the 72-h time-point, except NKT, where n=2 as only 2/5 donors had detectable levels of NKT cells. P-values from paired t-tests.