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. 2011 Aug 9;105(5):687–693. doi: 10.1038/bjc.2011.306

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Copyright © 2011 Cancer Research UK

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Effect of supernatants on HLA1 expression in tumour cells. Five tumour cell lines were cultured for 24 h with the supernatants derived from PBMCs treated with BCG (BCG-supernatant), before assessing HLA1 expression on the tumour cells. The basal levels of HLA1 expression expressed as MFI relative to the isotype control are presented in the text. The magnitudes of these expressions were compared with those on tumours after stimulation with supernatants from untreated PBMCs (CONT-supernatant). Data were presented as raw MFI (A) or relative to the respective controls (B). The relationship between HLA1 expression and cytotoxic effect of allogeneic CD8+ T-cells were assessed by measuring LDH activity (C). Effector:target ratios were at 20 : 1 and each data column represented the mean and s.d. of at least three separate experiments. P-values were from paired tests.