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. 2011 Aug 9;105(5):687–693. doi: 10.1038/bjc.2011.306

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Copyright © 2011 Cancer Research UK

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Effect of anti-IFN-γ and anti-TNF-α on HLA1 expression. A549, HCT116 and MCF7 cells were cultured with BCG supernatant in the presence or absence of neutralising antibodies against IFN-γ and TNF-α. Antagonising these cytokines caused reversal of the increases in HLA1 seen as a consequence of BCG-supernatant culture (A). Furthermore, culturing with the neutralising antibodies disrupted the extent of cytotoxicity seen with admixing with CD8+ T-cells (B). Each data column represents the mean and s.d. of at least three separate experiments.