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. 2011 Aug 9;20(21):4209–4223. doi: 10.1093/hmg/ddr348

Figure 2.

Figure 2.

Accumulation of secondary lysosomes in kidney proximal tubule epithelial cells and lamellar bodies in lung type II pneumocytes. (A) Light microscopy showing LAMP1 immunostaining (brown DAB staining) in kidney tubules of 1.5-month-old WT and KO mice. Electron microscopy showing kidney cortical proximal (p) and distal (d) tubules (B) and lung alveolar type II cells (C) from 6-month-old WT and 6- or 2-month-old KO mice. Male mice were used for analysis. (B, upper panel) Normal secondary lysosomes (dashed arrows) and enlarged secondary lysosomes with electron dense material (white arrows) and lipids (black arrows). V: enlarged apical vacuoles (phagosomes). (B: lower panel) Brush border microvilli (black arrows), normal secondary lysosomes (dashed arrows), enlarged secondary lysosomes with whorled membranes (white arrows) and lipids (L). V: apical vacuoles (phagosomes), N: cell nucleus. (C) Type II pneumocyte with normal lamellar bodies in WT lungs (white arrows) and enlarged lamellar bodies in KO lungs (black arrows). Dashed arrows indicate the surfactant. N: type II pneumocyte cell nuclei, NE: endothelial cell nucleus. Scale bars: 50 µm (A), 20 µm (B, upper panel), 2.5 µm (B, lower panel), 5 µm (C).