Table 3.
Primer Sequences Used to Synthesize cDNA Standards for Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction
| Gene | 3′ Primer | 5′ Primer |
|---|---|---|
| GAPDH | TCGACAGTCAGCCGCATCTTCT | ATGGCATGGACTGTGGTCAT |
| L13 | CCATCGTGGCTAAACAGGTA | TTTATTGGGCTCAGACCAGG |
| iNOS | AGAGTACAGCAAGTGGAAGT | TCCTGAACATAGACCTTGGG |
| ICAM-1 | CGAGGTGACCGTGAATGTG | AGTCTTGCTCCTTCCTCTTG |
| IFN-γ | GTGTGGAGACCATCAAGGAA | ACCCATGGGATCTTGCTTAG |
| TNF-α | GCCCGACTATCTCGACTT | GCTACATGGGAACAGCCTAT |
| IL-6 | TGAACTCCTTCTCCACAAGC | CCATGCTACATTTGCCGAAG |
| IL-12 | GACCAGCCTGGGAAACATAA | GCATCTGTCTGCTTCTCACA |
| Mac-1 | GCCGGTGAAATATGCTGTCTAC | TCCACTTTGGTCTCCGTCT |
| MMP-9 | AGGCGTCTGGACAAGCTG | CCAAGTTTATTAGAAACACTCCAAC |
| MMP-14 | GCAGCCTCTCACTACTCTTT | TGGGCAAGGTTCTTTCTCTG |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; iNOS, inducible nitric oxide synthase; ICAM-1, intercellular adhesion molecule–1; IFN-γ, inferferon-γ; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; Mac-1, macrophage-1 antigen; MMP-9, matrix metalloproteinase-9.