Skip to main content
. 2011 Sep 20;108(40):16681–16686. doi: 10.1073/pnas.1114209108

Fig. 3.

Fig. 3.

The DAFC-34B replication origin maps to the Vm34Ca gene. (A) Size-fractionated nascent DNA was isolated from stage 10 egg chambers, and the abundance of short nascent strands from the 1- to 1.5-kb fraction (plus λ-exonuclease treatment) was quantified by qPCR compared with a nonamplified locus. Genomic position is shown on the x-axis (8000 is Chr2L:13,408,000). The top of the figure shows the ORC2 ChIP on chip data for the region. There is a peak of nascent strand enrichment corresponding to the Vm34Ca transcription unit. (B) This enrichment is absent in the 5-kb and up fraction (minus λ-exonuclease treatment). (C) The region was assessed using additional primers in the 0.7- to 1-kb fraction (plus λ-exonuclease treatment). The enrichment was specific to the Vm34Ca gene and not to adjacent gene regions.