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A 6-kb ORC binding region is sufficient for amplification of DAFC-34B. (A) DNA sequences tested by P element-mediated transformation. (B) Results of ectopic amplification. Neither the 1-kb origin alone nor a 2.1-kb region containing the origin is sufficient for amplification. The 6-kb region containing the 1-kb origin as well as the peak of ORC binding is the minimal sequence that conferred amplification activity.
